Hyperpolarized xenon is an exquisite NMR probe for sensing molecular environments of the noble gas in solution. By trapping it in molecular cages like cryptophane-A, 129Xe can report information about molecular-specific binding events or resolve multiple signals simultaneously from different micro-environments in a lipid emulsion-a macroscopically- homogeneous phase that mimics properties of biological relevance. The Hyper-CEST detection scheme can be used in this context to pair significant signal enhancement with high specificity of xenon NMR resonances. Hyper-CEST can reduce the measurement time by a factor of up to 16 million and is currently able to detect biosensor concentrations as low as 1.4 nM. When combined with highly frequency-selective pulses, it also allows for demonstration of multiplexing potential using a single cage type as contrast agent for different environments in NMR imaging. This molecular imaging approach enables a switchable contrast that includes also temperature-sensitive imaging with molecular sensors that can be functionalized with various targeting molecules to bind, e.g., specifically to receptors of cancer cells. © 2009 Springer-Verlag.
CITATION STYLE
Schröder, L., Meldrum, T., Smith, M., Schilling, F., Denger, P., Zapf, S., … Pines, A. (2009). Xenon biosensors for multi-purpose molecular imaging. In IFMBE Proceedings (Vol. 25, pp. 176–179). Springer Verlag. https://doi.org/10.1007/978-3-642-03895-2_51
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