Diagnostic Accuracy of Loopamp Trypanosoma brucei Detection Kit for Diagnosis of Human African Trypanosomiasis in Clinical Samples

32Citations
Citations of this article
70Readers
Mendeley users who have this article in their library.

Abstract

Background:Molecular methods have great potential for sensitive parasite detection in the diagnosis of human African trypanosomiasis (HAT), but the requirements in terms of laboratory infrastructure limit their use to reference centres. A recently developed assay detects the Trypanozoon repetitive insertion mobile element (RIME) DNA under isothermal amplification conditions and has been transformed into a ready-to-use kit format, the Loopamp Trypanosoma brucei. In this study, we have evaluated the diagnostic performance of the Loopamp Trypanosoma brucei assay (hereafter called LAMP) in confirmed T.b. gambiense HAT patients, HAT suspects and healthy endemic controls from the Democratic Republic of the Congo (DRC).Methodology/Principal findings:142 T.b. gambiense HAT patients, 111 healthy endemic controls and 97 HAT suspects with unconfirmed status were included in this retrospective evaluation. Reference standard tests were parasite detection in blood, lymph or cerebrospinal fluid. Archived DNA from blood of all study participants was analysed in duplicate with LAMP. Sensitivity of LAMP in parasitologically confirmed cases was 87.3% (95% CI 80.9-91.8%) in the first run and 93.0% (95% CI 87.5-96.1%) in the second run. Specificity in healthy controls was 92.8% (95% CI 86.4-96.3%) in the first run and 96.4% (95% CI 91.1-98.6%) in the second run. Reproducibility was excellent with a kappa value of 0.81.Conclusions/Significance:In this laboratory-based study, the Loopamp Trypanosoma brucei Detection Kit showed good diagnostic accuracy and excellent reproducibility. Further studies are needed to assess the feasibility of its routine use for diagnosis of HAT under field conditions. © 2013 Mitashi et al.

References Powered by Scopus

An application of hierarchical Kappa type statistics in the assessment of majority agreement among multiple observers

2993Citations
N/AReaders
Get full text

Eliminating human African trypanosomiasis: Where do we stand and what comes next?

345Citations
N/AReaders
Get full text

Options for field diagnosis of human African trypanosomiasis

279Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Diagnostic testing for severe acute respiratory syndrome–related coronavirus 2: A narrative review

469Citations
N/AReaders
Get full text

Direct Blood Dry LAMP: A Rapid, Stable, and Easy Diagnostic Tool for Human African Trypanosomiasis

106Citations
N/AReaders
Get full text

Sensitivity and Specificity of a Prototype Rapid Diagnostic Test for the Detection of Trypanosoma brucei gambiense Infection: A Multi-centric Prospective Study

60Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Mitashi, P., Hasker, E., Ngoyi, D. M., Pyana, P. P., Lejon, V., Van der Veken, W., … Deborggraeve, S. (2013). Diagnostic Accuracy of Loopamp Trypanosoma brucei Detection Kit for Diagnosis of Human African Trypanosomiasis in Clinical Samples. PLoS Neglected Tropical Diseases, 7(10). https://doi.org/10.1371/journal.pntd.0002504

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 25

50%

Researcher 17

34%

Professor / Associate Prof. 4

8%

Lecturer / Post doc 4

8%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 15

41%

Biochemistry, Genetics and Molecular Bi... 8

22%

Immunology and Microbiology 8

22%

Medicine and Dentistry 6

16%

Article Metrics

Tooltip
Mentions
News Mentions: 1

Save time finding and organizing research with Mendeley

Sign up for free