Flux variability analysis enables comprehensive exploration of alternate optimal routes in a metabolic network. This method is especially useful with models such as bna572 for the developing oilseed rape embryo which is highly compartmentalized. Here, we describe a protocol for carrying out flux variability analysis on reactions and network projections of bna572 using well-established software (CellNetAnalyzer and COBRA) for constraint-based analysis of stoichiometric network reconstructions. © Springer Science+Business Media New York 2014.
CITATION STYLE
Hay, J. O., & Schwender, J. (2014). Flux variability analysis: Application to developing oilseed rape embryos using toolboxes for constraint-based modeling. Methods in Molecular Biology, 1090, 301–316. https://doi.org/10.1007/978-1-62703-688-7_18
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