Phenomic studies can provide a systemic overview of the network of interactions between phenotypes, genotypes, and environmental factors. Yeast (Saccharomyces cerevisiae) is one of the most important model organisms for phenomic studies due to the availability of a large variety of genome-wide strain collections. We describe a detailed protocol for performing a yeast phenomic screen for evaluation of protein colocalization via a genome-wide imaging-based screening approach utilizing a GFP-tagged yeast strain collection.
CITATION STYLE
Cao, X., Jin, X., & Liu, B. (2021). Genome-wide imaging-based phenomic screening using yeast (saccharomyces cerevisiae) strain collections. In Methods in Molecular Biology (Vol. 2196, pp. 85–95). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0868-5_8
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