Regulatory T cells are an important component of the immune system that plays a key role in maintaining homeostasis. Identification of distinct regulatory T cell subsets is essential to understand their function. Mass cytometry or CyTOF is a technology that enables the simultaneous measurement of up to 50 markers in single cells by using antibodies tagged with heavy metals, which are then detected with time-of-flight mass spectrometry. This chapter describes a mass cytometry approach for phenotypic characterization of regulatory T cells and determination of their master transcription factor Foxp3.
CITATION STYLE
Barcenilla, H., Pihl, M., Sjögren, F., Magnusson, L., & Casas, R. (2023). Regulatory T-Cell Phenotyping Using CyTOF. In Methods in Molecular Biology (Vol. 2559, pp. 231–242). Humana Press Inc. https://doi.org/10.1007/978-1-0716-2647-4_15
Mendeley helps you to discover research relevant for your work.