Microorganisms play a primary role in regulating biogeochemical cycles and are a valuable source of enzymes that have biotechnological applications, such as carbohydrate-active enzymes (CAZymes). However, the inability to culture the majority of microorganisms that exist in natural ecosystems restricts access to potentially novel bacteria and beneficial CAZymes. While commonplace molecular-based culture-independent methods such as metagenomics enable researchers to study microbial communities directly from environmental samples, recent progress in long-read sequencing technologies are advancing the field. We outline key methodological stages that are required as well as describe specific protocols that are currently used for long-read metagenomic projects dedicated to CAZyme discovery.
CITATION STYLE
Ferrillo, A., Kobel, C. M., Vera-Ponce de León, A., La Rosa, S. L., Kunath, B. J., Pope, P. B., & Hagen, L. H. (2023). Long-Read Metagenomics and CAZyme Discovery. Methods in Molecular Biology (Clifton, N.J.), 2657, 253–284. https://doi.org/10.1007/978-1-0716-3151-5_19
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