Molecular techniques such as real-time polymerase chain reaction (qPCR) have become a very effective alternative in food microbiology diagnostic for rapid and specific detection of foodborne pathogens such as Salmonella in foods and food-related environments. qPCR is a simple, sensitive, specific, and reproducible assay. Here, we describe the application of real-time PCR-based methods for a rapid (less than 24 h) detection of Salmonella in different types of foods fully compatible with the international standard for detection of Salmonella in food (ISO 6579-1:2017).
CITATION STYLE
Rodríguez-Lázaro, D., & Hernández, M. (2021). Next-Day Salmonella spp. Detection Method Based on Real-Time PCR for Foods. In Methods in Molecular Biology (Vol. 2182, pp. 1–6). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0791-6_1
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