Cell-based microarrays are promising tools for high-throughput functional analysis of gene products, but their application has been limited to adherent cells due to the difficulty in immobilization of non-adherent cells. Herein, we have introduced our techniques that can rapidly and strongly immobilize non-adherent cells and can allow the transfection of non-adherent cells with plasmid cDNA and small interfering RNA (siRNA) at a defined position on substrates with poly(ethylene-glycol)-lipid-modified surface.
CITATION STYLE
Yamaguchi, S., Matsunuma, E., & Nagamune, T. (2011). Immobilized culture and transfection microarray of non-adherent cells. In Methods in Molecular Biology (Vol. 706, pp. 151–157). Humana Press Inc. https://doi.org/10.1007/978-1-61737-970-3_12
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