Screening of protein crystallization conditions on a microfluidic chip using nanoliter-size droplets

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Abstract

Protein crystallization is a major bottleneck in determining tertiary protein structures from genomic sequence data. This paper describes a microfluidic system for screening hundreds of protein crystallization conditions using less than 4 nL of protein solution for each crystallization droplet. The droplets are formed by mixing protein, precipitant, and additive stock solutions in variable ratios in a flow of water-immiscible fluids inside microchannels. Each droplet represents a discrete trial testing different conditions. The system has been validated by crystallization of several water-soluble proteins. Copyright © 2003 American Chemical Society.

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Zheng, B., Roach, L. S., & Ismagilov, R. F. (2003). Screening of protein crystallization conditions on a microfluidic chip using nanoliter-size droplets. Journal of the American Chemical Society, 125(37), 11170–11171. https://doi.org/10.1021/ja037166v

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