Hyperspectral imaging — a novel concept for marker free chromosome characterization

Abstract

Hyperspectral Imaging (HSI) is a powerful and well-established technique. It combines different technologies like optical microscopy, digital imaging and molecular spectroscopy in combination with multivariate data analysis (MVDA) methods [1]. We describe the design and performance of our HSI System for Whiskbroom (single point scanning), Staring (2D wavelength scanning) and Pushbroom (line scanning with spectral dispersion) microspectroscopy. The system is optimized for the UV-VIS-NIR range and for fluorescence. In order to demonstrate the potential of the technique we will focus on the hyperspectral characterization of chromosomes.