Antioxidant activity of methanol extracts from Ganoderma lucidum Karst. Mycelia

Abstract

The study aimed to evaluate the effect of media cultivation on antioxidant activity of methanol extracts from Ganoderma lucidum mycelia. The effect of media was investigated in the submerged flask. Potatoes Dextrose Broth (PDB) and sweet corn were used as media, and cultivation incubation was carried out at 30°C. The results showed that the mean of mycelial dry weight, cultivated in different media were significantly different (p<0.05). The mycelia dry weight from sweet corn (0.221±0.004g) was higher than PDB media (0.199±0.008 g). Meanwhile to the metabolites content on the mycelia, the mycelia methanol extracts differed significantly (p<0.05). The total polyphenols of mycelia methanol extracts from PDB (13.152±1.89 GAE mg/g) was higher than mycelia methanol extracts from sweet corn (8.177±0.44 GAE mg/g). In line with total triterpenoids of mycelia methanol extracts from PDB and sweet corn with values of 33.085±2.95 mg/g and 11.199±2.74 mg/g, respectively. The scavenging to DPPH radical of methanol extract of mycelia which cultivated on PDB (22.140±3.25 %) higher than sweet corn media (12.840±2.03 %) at 0.2 mg/mL. The scavenging activity was suggested related to the presence of polyphenols and triterpenoids.