Inverted light-sheet microscopy (ILSM) is widely employed for fast large-volume imaging of biological tissue. However, the scattering especially in an uncleared sample, and the divergent propagation of the illumination beam lead to a trade-off between axial resolution and imaging depth. Herein, we propose naturally modulated ILSM (NM-ILSM) as a technique to improve axial resolution while simultaneously maintaining the wide field-of-view (FOV), and enhancing imaging contrast via background suppression. Theoretical derivations, simulations, and experimental imaging demonstrate 15% axial resolution increases, and fivefold greater image contrast compared with conventional ILSM. Therefore, NM-ILSM allows convenient imaging quality improvement for uncleared tissue and could extend the biological application scope of ILSM.
CITATION STYLE
Wang, Z., Qiao, W., Jiang, T., Chen, S., Lu, B., Ning, K., … Yuan, J. (2022). Axial resolution and imaging contrast enhancement in inverted light-sheet microscopy by natural illumination modulation. Frontiers in Neuroscience, 16. https://doi.org/10.3389/fnins.2022.1032195
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