Qualitative study of the new bone formation surrounding the ti-implant by FTIR and polarizing microscope

Abstract

Recently, dental implants have gained much attention for a great number of researchers in dental application. Until now, there are few reports about the properties and metabolism related to the bite force response of the newly bone formed surrounding the implants, which is poorly understood. For that reason, the purpose of this study was to elucidate the new bone formation surrounding the implants and its process of maturation by Micro-CT, polarizing microscope and FTIR analysis. Thus, the implants were inserted in the tibia of rabbits. After 4 and 8 weeks of surgical procedure, three-dimensional image of the implant and bone of non-decalcified samples was observed by Micro-CT analysis; bone structure and its maturity were seen by polarizing microscope; and finally, the PO43-, CCO32- and Amide I elements included in the interface boneimplant and in the new bone during the course time were examined by FTIR analysis. The results of this study, analyzed by this 3 methods, demonstrated that: 1) It was observed a qualitative difference in the new bone formation and the existing bone at 4 and 8 weeks of implantation; 2) There was also a qualitative difference in the newly bone formed at 4 and 8 weeks; 3) The maturation of the new bone started from the exhibiting bone; and 4) None of the methods used in current study destroyed the samples, and reproducibility results were obtained. In conclusion, we suggest a possible qualitative evaluation of new bone formation surroundings the Ti implant. ©2008 The Hard Tissue Biology Network Association Printed in Japan, All rights reserved.