A simple, selective, accurate, and precise high-performance thin-layer chromatographic (HPTLC) method has been established and validated for the analysis of artemisinin and its derivatives (artesunate, artemether, and arteether) in the bulk drugs and formulations. The artemisinin, artesunate, artemether, and arteether were separated on aluminum-backed silica gel 60 F 254 plates with toluene:ethyl acetate (10:1), toluene: ethyl acetate: acetic acid (2:8:0.2), toluene:butanol (10:1), and toluene:dichloro methane (0.5:10) mobile phase, respectively. The linear detector response for concentrations between 100 and 600 ng/spot showed good linear relationship with r value 0.9967, 0.9989, 0.9981 and 0.9989 for artemisinin, artesunate, artemether, and arteether, respectively. Statistical analysis proves that the method is precise, accurate, and reproducible and hence can be employed for the routine analysis. © 2011 Springer-Verlag Berlin Heidelberg.
CITATION STYLE
Agarwal, S. P., & Ahuja, S. (2011). HPTLC determination of artemisinin and its derivatives in bulk and pharmaceutical dosage. In High-Performance Thin-Layer Chromatography (HPTLC) (pp. 141–150). Springer Berlin Heidelberg. https://doi.org/10.1007/978-3-642-14025-9_9
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