Infection of ryegrass by three rust fungi (Puccinia coronata, P. graminis and P. loliina) and some effects of temperature on the establishment of the disease and sporulation

Abstract

Experiments were conducted to examine the processes leading up to the infection of Lolium temulentum by crown rust (Puccinia coronata), stem rust (P. graminis) and brown rust (P. loliina), and the effects of temperature on these processes and sporulation. Uredia of all three rusts were produced freely if the adaxial leaf surface was inoculated, but did not form following inoculation of the abaxial surface. Light and scanning electron microscopy revealed abnormal growth of germlings on the abaxial surface which had amorphous sheet-like epicuticular waxes and very few stomata. On the adaxial leaf surface germ tubes of all the rusts orientated at right angles to the long axis of the leaf. However, the directional growth of germ tubes was often disrupted when they contacted the surface of bulliform cells at the base of leaf grooves. For P. loliina the optimum temperatures for urediospore germination and sub-stomatal vesicle formation were 12-16°C, and 8-20°C for appressorium formation. The optimum temperatures, for the same stages of fungal development, for P. coronata and P. graminis were higher. Urediospore production of P. loliina was higher at 10°C than at 25°C, but was similar at both temperatures for P. coronata.