The isolation of recombinant antibodies by phage display naturally leads to experiments to evaluate their biological and immunological properties. Although crude preparations may have their value in initial studies, the need often exists for highly purified protein that can be tested in vivo. This chapter describes methods to generate high yields of scFv from bacterial cultures and to purify protein to the degree of homogeneity required for the most exacting analysis.
CITATION STYLE
Kipriyanov, S. M. (2009). High-level periplasmic expression and purification of scFvs. Methods in Molecular Biology (Clifton, N.J.), 562, 205–214. https://doi.org/10.1007/978-1-60327-302-2_16
Mendeley helps you to discover research relevant for your work.