MicroRNA Regulatory Network Analysis Using miRNet 2.0

Abstract

MicroRNAs exert their effects in the context of gene regulatory networks. The recent development of high-throughput experimental approaches and the growing availability of gene expression data have permitted comprehensive functional studies of miRNAs. However, the data interpretation is often challenging due to the fact that miRNAs not only act cooperatively with other miRNAs but also participate in complex networks by interacting with other functional elements, including non-coding RNAs or transcription factors that often have extensive effects on cell biology. This chapter provides detailed practical procedures on how to use miRNet 2.0 (https://www.mirnet.ca ) to perform miRNA regulatory network analytics to gain functional insights.