On‐line estimation of biological variables during pH controlled lactic acid fermentations

19Citations
Citations of this article
15Readers
Mendeley users who have this article in their library.
Get full text

Abstract

Indirect measurement of lactose, galactose, lactic acid, and biomass concentration from on‐line sodium hydroxide weight measurements have been obtained for pure and mixed batch cultures of Streptococcus salivarius ssp. thermophilus 404 and Lactobacillus delbrueckii subsp. bulgaricus 398 conducted at controlled pH and temperature. Linear correlations were established between the equivalent sodium hydroxide concentration and the lactose (substrate), galactose and lactic acid (products) concentrations while nonlinear relationships were developed between biomass and lactic acid concentrations. These nonlinear relationships took into account the inhibitory effect of lactic acid on growth and acidification. The indirect measurements of biomass concentration were introduced into a nonlinear estimator of the state variables and of the specific growth and lactic acid production rates. Good agreement was found between estimated and measured biomass concentrations (error index ranging from 10.8% to 12.6%). The results showed the feasibility of on‐line estimation of biomass concentration and of the specific kinetics from NaOH addition weight measurements and its applicability for monitoring lactic acid fermentations. Using off‐line measurements of L(+) and D(‐) lactic acid concentrations, the evolution of the concentration of each strain in mixed cultures was obtained from the relationships proposed for the mixed cultures. © 1994 John Wiley & Sons, Inc. Copyright © 1994 John Wiley & Sons, Inc.

References Powered by Scopus

On the practical identifiability of microbial growth models incorporating Michaelis-Menten type nonlinearities

216Citations
N/AReaders
Get full text

Studies on on‐line bioreactor identification. I. Theory

203Citations
N/AReaders
Get full text

Exocellular polysaccharide production by Streptococcus thermophilus

122Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Microbiological production of lactic acid

168Citations
N/AReaders
Get full text

Biomass measurement online: The performance of in situ measurements and software sensors

134Citations
N/AReaders
Get full text

Monitoring of submerged bioprocesses

56Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Acuña, G., Latrille, E., Béal, C., Corrieu, G., & Chéruy, A. (1994). On‐line estimation of biological variables during pH controlled lactic acid fermentations. Biotechnology and Bioengineering, 44(10), 1168–1176. https://doi.org/10.1002/bit.260441003

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 4

36%

Researcher 4

36%

Professor / Associate Prof. 2

18%

Lecturer / Post doc 1

9%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 7

64%

Engineering 2

18%

Chemical Engineering 1

9%

Chemistry 1

9%

Save time finding and organizing research with Mendeley

Sign up for free