The construction and transplantation of the tissue engineered cardiac conduction bundle

Abstract

In our study, we investigated whether the tissue engineered construct which composite of embryonic cardiac progenitor cells and collagen sponge can improve the atrioventricular block of rats. Cultured and identified the primary embryonic cardiac progenitor cells (ECPC), thenseeded into the collagen sponge to construct engineered tissues. Established the atrioventricular conduction block model of rats using the chemical method. The vivo experiments were grouped into sham group, empty scaffold group and constructs group. The state of the tissue engineered constructs in vivo was verified by using histomorphology. And Synchronization detection cardiac activity at different times. From the results, we can see that the constructs of ECPC and collagen sponge have good biocompatibility and express gap junction proteinsthrough histomorphology;the results of ECG showedthe complete atrioventricular block occurred; the construct group have the 80% recovery rate of surviving animals,the higher recovery rate and the longer implantion. Therefore, we can concluded that we can success to construct the tissue engineering construct cardiac conduction bundle by which the embryonic cardiac progenitor cells were seeded into the collagen sponge; ethanol injection can establish the rat model of atrioventricular block; Transplanted the tissue engineering construct cardiac conduction bundle can improve atrioventricular block.